Detailed information of TA system TA187759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4982046..4982648
• Replicon: chromosome
• Accession: NZ_CP068796
• Organism: Escherichia coli strain RIVM_C012087

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN38_RS23955
• Protein ID: WP_000897305.1
• Coordinates: 4982337..4982648 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN38_RS23950
• Protein ID: WP_000356395.1
• Coordinates: 4982046..4982336 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN38_RS23915	4977670..4978572	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN38_RS23920	4978569..4979204	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN38_RS23925	4979201..4980130	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN38_RS23930	4980312..4980554	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN38_RS23935	4980773..4980991	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN38_RS23940	4981410..4981688	-	279	WP_001315112.1	hypothetical protein	-
JNN38_RS23945	4981740..4981961	-	222	WP_001550354.1	hypothetical protein	-
JNN38_RS23950	4982046..4982336	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN38_RS23955	4982337..4982648	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN38_RS23960	4982877..4983785	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN38_RS23965	4983953..4985767	-	1815	WP_001550352.1	hypothetical protein	-
JNN38_RS23970	4986183..4987124	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN38_RS23975	4987169..4987606	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T187759 WP_000897305.1 NZ_CP068796:c4982648-4982337 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187759 NZ_CP089989:c1528131-1527598 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT187759 WP_000356395.1 NZ_CP068796:c4982336-4982046 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187759 NZ_CP089989:c1528408-1528142 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7