Detailed information of TA system TA187753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4066257..4066951
• Replicon: chromosome
• Accession: NZ_CP068796
• Organism: Escherichia coli strain RIVM_C012087

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN38_RS19615
• Protein ID: WP_001263491.1
• Coordinates: 4066257..4066655 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN38_RS19620
• Protein ID: WP_000554755.1
• Coordinates: 4066658..4066951 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN38_RS19585	4061426..4062670	-	1245	WP_000189541.1	esterase FrsA	-
-	4062086..4062166	-	81	NuclAT_10	-	-
-	4062086..4062166	-	81	NuclAT_10	-	-
-	4062086..4062166	-	81	NuclAT_10	-	-
-	4062086..4062166	-	81	NuclAT_10	-	-
JNN38_RS19590	4062762..4063220	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN38_RS19595	4063481..4064938	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN38_RS19600	4064995..4065347	-	353	Protein_3842	peptide chain release factor H	-
JNN38_RS19605	4065343..4065549	-	207	Protein_3843	RtcB family protein	-
JNN38_RS19610	4065795..4066247	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN38_RS19615	4066257..4066655	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN38_RS19620	4066658..4066951	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN38_RS19625	4067003..4068058	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN38_RS19630	4068129..4068914	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN38_RS19635	4068886..4070598	+	1713	Protein_3849	flagellar biosynthesis protein FlhA	-
JNN38_RS19640	4070703..4070981	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN38_RS19645	4070974..4071330	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4056193..4066951	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T187753 WP_001263491.1 NZ_CP068796:c4066655-4066257 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187753 NZ_CP089986:4025807-4026025 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT187753 WP_000554755.1 NZ_CP068796:c4066951-4066658 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187753 NZ_CP089986:4025406-4025780 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5