Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3840447..3840988 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag JNN54_RS18625 Protein ID WP_000615979.1
Coordinates 3840710..3840988 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JNN54_RS18620 Protein ID WP_000729704.1
Coordinates 3840447..3840707 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS18600 3836122..3836907 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
JNN54_RS18605 3836879..3838591 + 1713 Protein_3645 flagellar biosynthesis protein FlhA -
JNN54_RS18610 3838815..3839312 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JNN54_RS18615 3839488..3840261 - 774 WP_123297815.1 C40 family peptidase -
JNN54_RS18620 3840447..3840707 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JNN54_RS18625 3840710..3840988 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JNN54_RS18630 3841144..3841884 + 741 WP_001225679.1 murein L,D-transpeptidase -
JNN54_RS18635 3841855..3842622 - 768 WP_000333379.1 class II glutamine amidotransferase -
JNN54_RS18640 3842828..3843406 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T187728 WP_000615979.1 NZ_CP068795:3840710-3840988 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T187728 NZ_CP089976:c1960747-1960565 [Moraxella nasovis]
GTGCAAAGCCGTGTTATCATCAAAATGCTCATTGATGACGGTTGGTATGAAGTAAACGTAGTTGGTAGCCATCACCAATA
CAAACACCCCACTAAAAAGGGGAAAGTTACGGTAAAGCACCCTGACAAAGACATACCCATTGGCACCTACAAGAGCATTT
TAAAACAAGCTGGGTTAAACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT187728 WP_000729704.1 NZ_CP068795:3840447-3840707 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT187728 NZ_CP089976:c1960532-1960122 [Moraxella nasovis]
ATGTATTACCCCATCGCCATTGAAAGCCCTGCCAATGATAATGAATGTTTTGGCGTGATTGTCCCTGACTTACCAGGCTG
TTTTAGTGCAGGAAACAGTTTAGATGATGCCATCAAAAGCGTTCATGAAGCCATCAGCCTACACCTTGACGGATTAGCAA
AGGACGGTGATGACATTCCACTTGCCAAAAATGTTGCAGACCATGCAAAAAACCCAGACTATCAAGGCATGACATGGGCG
GTGGTAGATATTGACCTATCTGCCTATATGGGTAAAAGCGAAAAAATCAACGTAACCTTGCCATCTCGTCTGATTGCACG
GATTGATGAAAAAGTATCCGCTCATAAATCGCTATATAAAAGCCGTAGCAACTATTTGGCGCAGCTTGCCAGTCGTGATT
TGATGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References