Detailed information of TA system TA187727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3834250..3834944
• Replicon: chromosome
• Accession: NZ_CP068795
• Organism: Escherichia coli strain RIVM_C011532

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JNN54_RS18585
• Protein ID: WP_001263493.1
• Coordinates: 3834250..3834648 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JNN54_RS18590
• Protein ID: WP_000554757.1
• Coordinates: 3834651..3834944 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN54_RS18555	3829250..3830494	-	1245	WP_000189541.1	esterase FrsA	-
-	3829910..3829990	-	81	NuclAT_11	-	-
-	3829910..3829990	-	81	NuclAT_11	-	-
-	3829910..3829990	-	81	NuclAT_11	-	-
-	3829910..3829990	-	81	NuclAT_11	-	-
JNN54_RS18560	3830586..3831044	-	459	WP_061349133.1	xanthine phosphoribosyltransferase	-
JNN54_RS18565	3831305..3832762	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JNN54_RS18570	3832819..3833340	-	522	Protein_3638	peptide chain release factor H	-
JNN54_RS18575	3833339..3833542	-	204	Protein_3639	RNA ligase RtcB family protein	-
JNN54_RS18580	3833788..3834240	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JNN54_RS18585	3834250..3834648	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN54_RS18590	3834651..3834944	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN54_RS18595	3834996..3836051	-	1056	WP_001226164.1	DNA polymerase IV	-
JNN54_RS18600	3836122..3836907	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
JNN54_RS18605	3836879..3838591	+	1713	Protein_3645	flagellar biosynthesis protein FlhA	-
JNN54_RS18610	3838815..3839312	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T187727 WP_001263493.1 NZ_CP068795:c3834648-3834250 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187727 NZ_CP089976:865561-865722 [Moraxella nasovis]
GTGCAAAGCCGTGTTATCATCAAAATGCTCATTGATGACGGTTGGTATGAAGTAAACGTAGTTGGTAGCCATCACCAATA
CAAACACCCCACTAAAAAGGGGAAAGTTACGGTAAAGCACCCTGACAAAGACATACCCATTGGCACCTACAAGAGCATTT
AA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT187727 WP_000554757.1 NZ_CP068795:c3834944-3834651 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187727 NZ_CP089976:865775-866185 [Moraxella nasovis]
ATGTATTACCCCATCGCCATTGAAAGCCCTGCCAATGATAATGAATGTTTTGGCGTGATTGTCCCTGACTTACCAGGCTG
TTTTAGTGCAGGAAACAGTTTAGATGATGCCATCAAAAGCGTTCATGAAGCCATCAGCCTACACCTTGACGGATTAGCAA
AGGACGGTGATGACATTCCACTTGCCAAAAATGTTGCAGACCATGCAAAAAACCCAGACTATCAAGGCATGACATGGGCG
GTGGTAGATATTGACCTATCTGCCTATATGGGTAAAAGCGAAAAAATCAACGTAACCTTGCCATCTCGTCTGATTGCACG
GATTGATGAAAAAGTATCCGCTCATAAATCGCTATATAAAAGCCGTAGCAACTATTTGGCACAGCTTGCCAGTCGTGATT
TGATGGCGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6