Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1870988..1871490 Replicon chromosome
Accession NZ_CP068795
Organism Escherichia coli strain RIVM_C011532

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN54_RS08860 Protein ID WP_000767829.1
Coordinates 1871236..1871490 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN54_RS08855 Protein ID WP_001259255.1
Coordinates 1870988..1871239 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN54_RS08830 1866165..1867232 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN54_RS08835 1867232..1868302 - 1071 WP_072649825.1 histidinol-phosphate transaminase -
JNN54_RS08840 1868299..1869603 - 1305 WP_001446927.1 histidinol dehydrogenase -
JNN54_RS08845 1869609..1870508 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JNN54_RS08850 1870654..1870704 - 51 WP_001364200.1 his operon leader peptide -
JNN54_RS08855 1870988..1871239 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN54_RS08860 1871236..1871490 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN54_RS08865 1871573..1872397 + 825 WP_038989073.1 SDR family oxidoreductase -
JNN54_RS08870 1872443..1873372 + 930 WP_064221651.1 LysR family transcriptional regulator -
JNN54_RS08875 1873587..1873649 + 63 WP_010723108.1 membrane protein YoeI -
JNN54_RS08880 1873639..1874997 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN54_RS08885 1875177..1876235 + 1059 WP_000492330.1 transport protein YeeE -
JNN54_RS08890 1876249..1876476 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T187713 WP_000767829.1 NZ_CP068795:1871236-1871490 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T187713 NZ_CP089960:192035-192319 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT187713 WP_001259255.1 NZ_CP068795:1870988-1871239 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT187713 NZ_CP089960:191797-192045 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References