Detailed information of TA system TA187703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4509633..4510285
• Replicon: chromosome
• Accession: NZ_CP068771
• Organism: Serratia plymuthica strain UBCF_13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JK621_RS21020
• Protein ID: WP_212557500.1
• Coordinates: 4509941..4510285 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2X4V2M0
• Locus tag: JK621_RS21015
• Protein ID: WP_063200237.1
• Coordinates: 4509633..4509935 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK621_RS20995 (JK621_20995)	4504912..4505820	+	909	WP_212557496.1	LysR family transcriptional regulator	-
JK621_RS21000 (JK621_21000)	4506136..4507290	+	1155	WP_212557497.1	MFS transporter	-
JK621_RS21005 (JK621_21005)	4507280..4508203	-	924	WP_212557498.1	LysR family transcriptional regulator	-
JK621_RS21010 (JK621_21010)	4508340..4509602	+	1263	WP_212557499.1	diaminopimelate decarboxylase	-
JK621_RS21015 (JK621_21015)	4509633..4509935	-	303	WP_063200237.1	XRE family transcriptional regulator	Antitoxin
JK621_RS21020 (JK621_21020)	4509941..4510285	-	345	WP_212557500.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK621_RS21025 (JK621_21025)	4510424..4511434	-	1011	WP_212557501.1	LacI family DNA-binding transcriptional regulator	-
JK621_RS21030 (JK621_21030)	4511586..4512041	+	456	WP_212557502.1	GNAT family N-acetyltransferase	-
JK621_RS21035 (JK621_21035)	4512038..4513057	-	1020	WP_212557503.1	HTH-type transcriptional regulator GalR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13257.26 Da
• Isoelectric Point: 9.0591

>T187703 WP_212557500.1 NZ_CP068771:c4510285-4509941 [Serratia plymuthica]
MWQVVTVERFDDGFLSLNGDEQKSVLAEIFKLQEFGPLLARPHADTLHFSSSIRHLKELRIQHRGRPFRVFFAFDPQHLA
VLLCGGDKTGDKRFYQRLLPIAAMEFSHYLATQR

Nucleotide

• Length: 345 bp

>T187703 NZ_CP089959:794470-794880 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11126.05 Da
• Isoelectric Point: 10.1425

>AT187703 WP_063200237.1 NZ_CP068771:c4509935-4509633 [Serratia plymuthica]
MAKPLSNLIDKLDPTVVAAARQKAEKEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLEVELPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT187703 NZ_CP089959:794223-794489 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X4V2M0