Detailed information of TA system TA187646

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3883255..3883667
• Replicon: chromosome
• Accession: NZ_CP068706
• Organism: Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: JK375_RS18940
• Protein ID: WP_000132601.1
• Coordinates: 3883326..3883667 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3883255..3883331 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK375_RS18930	3880118..3881707	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
JK375_RS18935	3881704..3883098	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3883255..3883331	-	77	NuclAT_14	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_14	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_14	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_14	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_15	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_15	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_15	-	Antitoxin
-	3883255..3883331	-	77	NuclAT_15	-	Antitoxin
JK375_RS18940	3883326..3883667	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
JK375_RS18945	3883829..3885208	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
JK375_RS18950	3885208..3886254	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T187646 WP_000132601.1 NZ_CP068706:3883326-3883667 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T187646 NZ_CP089932:4844981-4845265 [Erwinia tracheiphila]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCTGC
AATTGAAGAAGATGATACAAAATACCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATGAGTCAGCTATCTA
AAGAAGTTGGCATGTCCAGAGAGGGAATTTACAAGGCGTTATCCGGTGAAGGGAATCCATCATTCCAGACTGTTTCTAAA
ATCACAAAAGCGCTAGGCTTAAAACTTCACTTCACAGGGGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT187646 NZ_CP068706:c3883331-3883255 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin