Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3568816..3569510 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JK375_RS17465 Protein ID WP_001263489.1
Coordinates 3568816..3569214 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JK375_RS17470 Protein ID WP_000554758.1
Coordinates 3569217..3569510 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3564404..3564484 - 81 NuclAT_12 - -
- 3564404..3564484 - 81 NuclAT_12 - -
- 3564404..3564484 - 81 NuclAT_12 - -
- 3564404..3564484 - 81 NuclAT_12 - -
JK375_RS17440 3565080..3565538 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JK375_RS17445 3565799..3567256 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JK375_RS17450 3567313..3567834 - 522 Protein_3413 peptide chain release factor H -
JK375_RS17455 3567830..3568036 - 207 Protein_3414 RtcB family protein -
JK375_RS17460 3568354..3568806 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
JK375_RS17465 3568816..3569214 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JK375_RS17470 3569217..3569510 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JK375_RS17475 3569562..3570617 - 1056 WP_001226164.1 DNA polymerase IV -
JK375_RS17480 3570688..3571473 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JK375_RS17485 3571445..3573157 + 1713 Protein_3420 flagellar biosynthesis protein FlhA -
JK375_RS17490 3573381..3573878 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T187644 WP_001263489.1 NZ_CP068706:c3569214-3568816 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T187644 NZ_CP089932:4843060-4843350 [Erwinia tracheiphila]
CTGCCGGGTGATCCACTTGGAAAATTCACCTTTAAAAAGCGCCTCGGCTTGCCCGGAGTAGGAGCCAGAGATGGAGCACG
ATCAATCGTTTTTTTCAATGATGGCGACAACATCTTCTTTTTTGATATGTATCTGAAAAGTGGGCTTTCAAAAAAGAAAG
GAAAAGAGCTTGAAGATGATGAAATTGATGCTTACTGCAATATCGCAAAAGATTTTATCTCAATGAGTCCGGCAACTATA
AATAAGCTTCTGTCTGATAAAGAACTGATCGAGGTAAATTGTGATGACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT187644 WP_000554758.1 NZ_CP068706:c3569510-3569217 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT187644 NZ_CP089932:4843343-4843660 [Erwinia tracheiphila]
ATGACTGAACGTCTGAAACGCATTCAAGGTATGGCTGAACGCTATAACAAACTAGGCGTGGTAAAAGATGAAACCGTAGC
TGCAATTGATGCAGCGGTCGCAGCCCGTAATATCCCGGCGATCCGAGTTATGACCGGAAGCCAGATAAAGGAAGTCCGCA
CTCGCTACAACATGTCGCAAGCCGCATTAGCTATAACAACCGGCATGTCTGTAGAAAGCGTCTCCAAATGGGAGCGAAAC
GAAAGCAAACCGAATAAGGCTGCTCTGCGGATCATCAACATCATCGATGCTAAAGGGCCACAGGTGTTCATGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References