Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1695863..1696365 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JK375_RS08080 Protein ID WP_000767829.1
Coordinates 1696111..1696365 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JK375_RS08075 Protein ID WP_001259255.1
Coordinates 1695863..1696114 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS08050 1691041..1692108 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JK375_RS08055 1692108..1693178 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JK375_RS08060 1693175..1694479 - 1305 WP_000009594.1 histidinol dehydrogenase -
JK375_RS08065 1694485..1695384 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JK375_RS08070 1695530..1695580 - 51 WP_001364200.1 his operon leader peptide -
JK375_RS08075 1695863..1696114 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JK375_RS08080 1696111..1696365 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JK375_RS08085 1696448..1697272 + 825 WP_000754737.1 SDR family oxidoreductase -
JK375_RS08090 1697318..1698247 + 930 WP_000803351.1 LysR family transcriptional regulator -
JK375_RS08095 1698462..1698524 + 63 WP_010723108.1 membrane protein YoeI -
JK375_RS08100 1698514..1699872 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JK375_RS08105 1700051..1701109 + 1059 WP_000492339.1 transport protein YeeE -
JK375_RS08110 1701123..1701350 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T187630 WP_000767829.1 NZ_CP068706:1696111-1696365 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T187630 NZ_CP089927:c4088160-4087777 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT187630 WP_001259255.1 NZ_CP068706:1695863-1696114 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT187630 NZ_CP089927:c4088410-4088255 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References