Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 505221..506020 Replicon chromosome
Accession NZ_CP068706
Organism Escherichia coli strain J53-p1-pV-hybrid-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JK375_RS02520 Protein ID WP_000347273.1
Coordinates 505221..505685 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JK375_RS02525 Protein ID WP_001307405.1
Coordinates 505685..506020 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK375_RS02495 501397..501954 - 558 Protein_488 amidohydrolase family protein -
JK375_RS02500 501950..502321 - 372 Protein_489 PTS sugar transporter subunit IIC -
JK375_RS02505 502332..502805 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JK375_RS02510 502828..504108 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JK375_RS02515 504357..505166 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JK375_RS02520 505221..505685 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JK375_RS02525 505685..506020 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JK375_RS02530 506169..507740 - 1572 WP_001273753.1 galactarate dehydratase -
JK375_RS02535 508115..509449 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JK375_RS02540 509465..510235 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T187623 WP_000347273.1 NZ_CP068706:c505685-505221 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T187623 NZ_CP089927:4012723-4013130 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT187623 WP_001307405.1 NZ_CP068706:c506020-505685 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187623 NZ_CP089927:4012511-4012726 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References