Detailed information of TA system TA187592

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 162820..163073
• Replicon: plasmid pRJBSI76-3
• Accession: NZ_CP068692
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I5002_RS30425
• Protein ID: WP_001312851.1
• Coordinates: 162820..162969 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	163014..163073 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS30390	158179..158594	-	416	Protein_215	IS1 family transposase	-
I5002_RS30395	158843..159244	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I5002_RS30400	159177..159434	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I5002_RS30405	159527..160180	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I5002_RS30410	161119..161976	-	858	WP_159194827.1	incFII family plasmid replication initiator RepA	-
I5002_RS30415	161969..162043	-	75	WP_001375168.1	RepA leader peptide Tap	-
I5002_RS30420	162288..162536	-	249	WP_000083837.1	replication regulatory protein RepA	-
I5002_RS30425	162820..162969	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	163014..163073	+	60	NuclAT_1	-	Antitoxin
-	163014..163073	+	60	NuclAT_1	-	Antitoxin
-	163014..163073	+	60	NuclAT_1	-	Antitoxin
-	163014..163073	+	60	NuclAT_1	-	Antitoxin
I5002_RS30430	163274..163504	-	231	WP_001736714.1	hypothetical protein	-
I5002_RS30435	163668..164267	-	600	WP_032083981.1	hypothetical protein	-
I5002_RS30440	164653..164853	-	201	WP_015059022.1	hypothetical protein	-
I5002_RS30445	164985..165545	-	561	WP_000139328.1	fertility inhibition protein FinO	-
I5002_RS30450	165600..166346	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
I5002_RS30455	166366..166527	-	162	Protein_228	DNA helicase	-
I5002_RS30460	166591..167295	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I5002_RS30465	167348..167413	+	66	Protein_230	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B	-	1..184748	184748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T187592 WP_001312851.1 NZ_CP068692:c162969-162820 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T187592 NZ_CP089927:c1154972-1154412 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Nucleotide

• Length: 60 bp

>AT187592 NZ_CP068692:163014-163073 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin