Detailed information of TA system TA187588

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131497..131923
• Replicon: plasmid pRJBSI76-3
• Accession: NZ_CP068692
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I5002_RS30220
• Protein ID: WP_001312861.1
• Coordinates: 131497..131655 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	131699..131923 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5002_RS30185	126551..126778	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
I5002_RS30190	126873..127559	-	687	WP_015059009.1	PAS domain-containing protein	-
I5002_RS30195	127750..128133	-	384	WP_000124981.1	relaxosome protein TraM	-
I5002_RS30200	128410..129057	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
I5002_RS30205	129354..130175	-	822	WP_001234445.1	DUF945 domain-containing protein	-
I5002_RS30210	130286..130582	-	297	WP_001272251.1	hypothetical protein	-
I5002_RS30215	130882..131259	+	378	Protein_180	hypothetical protein	-
I5002_RS30220	131497..131655	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	131699..131923	-	225	NuclAT_0	-	Antitoxin
-	131699..131923	-	225	NuclAT_0	-	Antitoxin
-	131699..131923	-	225	NuclAT_0	-	Antitoxin
-	131699..131923	-	225	NuclAT_0	-	Antitoxin
I5002_RS30225	131935..132654	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
I5002_RS30230	132651..133085	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I5002_RS30235	133154..135177	-	2024	Protein_184	ParB/RepB/Spo0J family partition protein	-
I5002_RS30240	135238..135471	-	234	WP_000006003.1	DUF905 family protein	-
I5002_RS30245	135529..136056	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B	-	1..184748	184748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T187588 WP_001312861.1 NZ_CP068692:c131655-131497 [Klebsiella pneumoniae subsp. pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T187588 NZ_CP089927:c1116645-1116316 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT187588 NZ_CP068692:c131923-131699 [Klebsiella pneumoniae subsp. pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin