Detailed information of TA system TA187559

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 162823..163076
• Replicon: plasmid pRJBSI76-pV-3
• Accession: NZ_CP068687
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JMY88_RS30365
• Protein ID: WP_001312851.1
• Coordinates: 162823..162972 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	163017..163076 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS30330	158182..158597	-	416	Protein_215	IS1 family transposase	-
JMY88_RS30335	158846..159247	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JMY88_RS30340	159180..159437	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JMY88_RS30345	159530..160183	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JMY88_RS30350	161122..161979	-	858	WP_159194827.1	incFII family plasmid replication initiator RepA	-
JMY88_RS30355	161972..162046	-	75	WP_001375168.1	RepA leader peptide Tap	-
JMY88_RS30360	162291..162539	-	249	WP_000083837.1	replication regulatory protein RepA	-
JMY88_RS30365	162823..162972	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	163017..163076	+	60	NuclAT_1	-	Antitoxin
-	163017..163076	+	60	NuclAT_1	-	Antitoxin
-	163017..163076	+	60	NuclAT_1	-	Antitoxin
-	163017..163076	+	60	NuclAT_1	-	Antitoxin
JMY88_RS30370	163277..163507	-	231	WP_001736714.1	hypothetical protein	-
JMY88_RS30375	163671..164270	-	600	WP_032083981.1	hypothetical protein	-
JMY88_RS30380	164656..164856	-	201	WP_015059022.1	hypothetical protein	-
JMY88_RS30385	164988..165548	-	561	WP_000139328.1	fertility inhibition protein FinO	-
JMY88_RS30390	165603..166349	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JMY88_RS30395	166369..166530	-	162	Protein_228	DNA helicase	-
JMY88_RS30400	166594..167298	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JMY88_RS30405	167351..167416	+	66	Protein_230	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B	-	1..184751	184751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T187559 WP_001312851.1 NZ_CP068687:c162972-162823 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T187559 NZ_CP089919:c2509762-2509484 [Rahnella victoriana]
ATGATTAAGGATTTTAAACATAAAGGACTGCGCCGGTTTTTCACTTCGGGTTCAACCGCAGGCATTAATGCGGAACACGC
AAAAAAACTCAGACTCAGGCTTGCCACCCTCCATTCGGCTCAGGCTGTTCAGGATATTGACCGGCCTGGCTATTTGTTGC
ACGCCCTCGCGGGTTCGCGAAAGGGGGAATGGGCAATAACGGTCTCTGGCAACTGGCGTCTTACCTTTGAATTTATCGAT
GGAAATGCTTACATCGTCAATTATGAGGATTATCACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT187559 NZ_CP068687:163017-163076 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin