Detailed information of TA system TA187555

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131500..131926
• Replicon: plasmid pRJBSI76-pV-3
• Accession: NZ_CP068687
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JMY88_RS30160
• Protein ID: WP_001312861.1
• Coordinates: 131500..131658 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	131702..131926 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS30125	126554..126781	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JMY88_RS30130	126876..127562	-	687	WP_015059009.1	PAS domain-containing protein	-
JMY88_RS30135	127753..128136	-	384	WP_000124981.1	relaxosome protein TraM	-
JMY88_RS30140	128413..129060	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JMY88_RS30145	129357..130178	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JMY88_RS30150	130289..130585	-	297	WP_001272251.1	hypothetical protein	-
JMY88_RS30155	130885..131262	+	378	Protein_180	hypothetical protein	-
JMY88_RS30160	131500..131658	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
-	131702..131926	-	225	NuclAT_0	-	Antitoxin
JMY88_RS30165	131938..132657	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JMY88_RS30170	132654..133088	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JMY88_RS30175	133157..135180	-	2024	Protein_184	ParB/RepB/Spo0J family partition protein	-
JMY88_RS30180	135241..135474	-	234	WP_000006003.1	DUF905 family protein	-
JMY88_RS30185	135532..136059	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B	-	1..184751	184751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T187555 WP_001312861.1 NZ_CP068687:c131658-131500 [Klebsiella pneumoniae subsp. pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T187555 NZ_CP089919:c31466-31377 [Rahnella victoriana]
GCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATCGTATTCG
GTCTTTTTTT

Antitoxin

Nucleotide

• Length: 225 bp

>AT187555 NZ_CP068687:c131926-131702 [Klebsiella pneumoniae subsp. pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin