Detailed information of TA system TA187552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 184820..185547
• Replicon: plasmid pRJBSI76-pV-2
• Accession: NZ_CP068686
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JMY88_RS29015
• Protein ID: WP_011251285.1
• Coordinates: 184820..185131 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JMY88_RS29020
• Protein ID: WP_011251286.1
• Coordinates: 185128..185547 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS28990	179858..181204	-	1347	WP_011154555.1	dihydroorotase	-
JMY88_RS28995	181201..182052	-	852	WP_004214538.1	M14 family metallocarboxypeptidase	-
JMY88_RS29000	182045..183046	-	1002	WP_011251272.1	TGS domain-containing protein	-
JMY88_RS29005	183205..183902	-	698	Protein_191	IS1 family transposase	-
JMY88_RS29010	184157..184615	+	459	Protein_192	DDE-type integrase/transposase/recombinase	-
JMY88_RS29015	184820..185131	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JMY88_RS29020	185128..185547	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JMY88_RS29025	185694..186662	+	969	WP_074428168.1	IS5 family transposase	-
JMY88_RS29030	186764..187099	-	336	Protein_196	conjugal transfer protein TraI	-
JMY88_RS29035	187113..187901	-	789	WP_040217257.1	hypothetical protein	-
JMY88_RS29040	187922..188365	-	444	WP_048333569.1	hypothetical protein	-
JMY88_RS29045	188961..189716	+	756	Protein_199	hypothetical protein	-
JMY88_RS29050	189909..190379	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(7'')-Ia	iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..225445	225445
	-	inside	IScluster/Tn	-	-	176979..195683	18704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T187552 WP_011251285.1 NZ_CP068686:184820-185131 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T187552 NZ_CP089884:c5296110-5295727 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATACCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT187552 WP_011251286.1 NZ_CP068686:185128-185547 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT187552 NZ_CP089884:c5296352-5296110 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1