Detailed information of TA system TA187549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 206313..207040
• Replicon: plasmid pRJBSI76-pV-1
• Accession: NZ_CP068685
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JMY88_RS27775
• Protein ID: WP_011251285.1
• Coordinates: 206729..207040 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JMY88_RS27770
• Protein ID: WP_011251286.1
• Coordinates: 206313..206732 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY88_RS27740	201481..201951	-	471	WP_048333570.1	hypothetical protein	-
JMY88_RS27745	202144..202899	-	756	Protein_216	hypothetical protein	-
JMY88_RS27750	203495..203938	+	444	WP_048333569.1	hypothetical protein	-
JMY88_RS27755	203959..204747	+	789	WP_040217257.1	hypothetical protein	-
JMY88_RS27760	204761..205096	+	336	Protein_219	conjugal transfer protein TraI	-
JMY88_RS27765	205198..206166	-	969	WP_011251287.1	IS5 family transposase	-
JMY88_RS27770	206313..206732	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JMY88_RS27775	206729..207040	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JMY88_RS27780	207245..207703	-	459	Protein_223	DDE-type integrase/transposase/recombinase	-
JMY88_RS27785	207958..208655	+	698	Protein_224	IS1 family transposase	-
JMY88_RS27790	208814..209815	+	1002	WP_011251272.1	TGS domain-containing protein	-
JMY88_RS27795	209808..210659	+	852	WP_004214538.1	M14 family metallocarboxypeptidase	-
JMY88_RS27800	210656..212002	+	1347	WP_011154555.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..263776	263776
	-	inside	IScluster/Tn	-	-	196177..214881	18704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T187549 WP_011251285.1 NZ_CP068685:c207040-206729 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T187549 NZ_CP089884:c4670910-4670377 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT187549 WP_011251286.1 NZ_CP068685:c206732-206313 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT187549 NZ_CP089884:c4671187-4670921 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1