Detailed information of TA system TA187512

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75112..75381
• Replicon: plasmid unnamed
• Accession: NZ_CP068681
• Organism: Escherichia coli strain NCCP12480

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: JKV47_RS22330
• Protein ID: WP_001323520.1
• Coordinates: 75265..75381 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75112..75177 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKV47_RS22280	70493..70662	+	170	Protein_70	hypothetical protein	-
JKV47_RS22285	70845..70925	-	81	Protein_71	hypothetical protein	-
JKV47_RS22290	70995..71201	+	207	WP_000275856.1	hypothetical protein	-
JKV47_RS22295	71227..71766	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
JKV47_RS22300	71834..72067	+	234	WP_000005990.1	DUF905 family protein	-
JKV47_RS22305	72095..72292	+	198	Protein_75	hypothetical protein	-
JKV47_RS22310	72347..72781	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JKV47_RS22315	72778..73540	+	763	Protein_77	plasmid SOS inhibition protein A	-
-	73509..73611	+	103	NuclAT_1	-	-
-	73509..73611	+	103	NuclAT_1	-	-
-	73509..73611	+	103	NuclAT_1	-	-
-	73509..73611	+	103	NuclAT_1	-	-
JKV47_RS22320	73657..75026	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	75053..75179	+	127	NuclAT_0	-	-
-	75053..75179	+	127	NuclAT_0	-	-
-	75053..75179	+	127	NuclAT_0	-	-
-	75053..75179	+	127	NuclAT_0	-	-
-	75112..75177	-	66	-	-	Antitoxin
JKV47_RS22325	75165..75314	+	150	Protein_79	plasmid maintenance protein Mok	-
JKV47_RS22330	75265..75381	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
JKV47_RS22335	75601..75831	+	231	WP_071586998.1	hypothetical protein	-
JKV47_RS22340	75829..76001	-	173	Protein_82	hypothetical protein	-
JKV47_RS22345	76071..76277	+	207	WP_000547968.1	hypothetical protein	-
JKV47_RS22350	76302..76589	+	288	WP_000107535.1	hypothetical protein	-
JKV47_RS22355	76707..77528	+	822	WP_001234426.1	DUF932 domain-containing protein	-
JKV47_RS22360	77825..78427	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JKV47_RS22365	78748..79131	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JKV47_RS22370	79318..80007	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..82394	82394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T187512 WP_001323520.1 NZ_CP068681:75265-75381 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T187512 NZ_CP089877:c5187385-5187002 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT187512 NZ_CP068681:c75177-75112 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin