Detailed information of TA system TA187489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 65816..66510
• Replicon: chromosome
• Accession: NZ_CP068680
• Organism: Escherichia coli strain NCCP12480

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JKV47_RS00345
• Protein ID: WP_001263493.1
• Coordinates: 65816..66214 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JKV47_RS00350
• Protein ID: WP_000554757.1
• Coordinates: 66217..66510 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (61476)	61476..61556	-	81	NuclAT_13	-	-
- (61476)	61476..61556	-	81	NuclAT_13	-	-
- (61476)	61476..61556	-	81	NuclAT_13	-	-
- (61476)	61476..61556	-	81	NuclAT_13	-	-
JKV47_RS00315 (60816)	60816..62060	-	1245	WP_000189541.1	esterase FrsA	-
JKV47_RS00320 (62152)	62152..62610	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JKV47_RS00325 (62871)	62871..64328	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JKV47_RS00330 (64385)	64385..64906	-	522	Protein_64	peptide chain release factor H	-
JKV47_RS00335 (64905)	64905..65108	-	204	Protein_65	RtcB family protein	-
JKV47_RS00340 (65354)	65354..65806	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JKV47_RS00345 (65816)	65816..66214	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JKV47_RS00350 (66217)	66217..66510	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JKV47_RS00355 (66562)	66562..67617	-	1056	WP_001226164.1	DNA polymerase IV	-
JKV47_RS00360 (67688)	67688..68473	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
JKV47_RS00365 (68445)	68445..70157	+	1713	Protein_71	flagellar biosynthesis protein FlhA	-
JKV47_RS00370 (70373)	70373..70870	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T187489 WP_001263493.1 NZ_CP068680:c66214-65816 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187489 NZ_CP089874:351689-352036 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT187489 WP_000554757.1 NZ_CP068680:c66510-66217 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187489 NZ_CP089874:352036-352335 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6