Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 431436..432031 Replicon chromosome
Accession NZ_CP068678
Organism Pseudomonas aeruginosa strain NCCP15783

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag JKV45_RS02135 Protein ID WP_003113526.1
Coordinates 431753..432031 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JKV45_RS02130 Protein ID WP_003113527.1
Coordinates 431436..431741 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKV45_RS02095 (JKV45_02095) 426577..427425 + 849 WP_003099284.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
JKV45_RS02105 (JKV45_02105) 427592..428533 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
JKV45_RS02110 (JKV45_02110) 428650..429264 + 615 WP_003099279.1 50S ribosomal protein L25/general stress protein Ctc -
JKV45_RS02115 (JKV45_02115) 429306..429890 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
JKV45_RS02120 (JKV45_02120) 429931..431031 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
JKV45_RS02130 (JKV45_02130) 431436..431741 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
JKV45_RS02135 (JKV45_02135) 431753..432031 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKV45_RS02140 432084..432212 - 129 Protein_422 integrase -
JKV45_RS02145 (JKV45_02140) 432360..434588 + 2229 WP_003099265.1 TonB-dependent receptor -
JKV45_RS02150 (JKV45_02145) 434658..435305 - 648 WP_003095021.1 carbonate dehydratase -
JKV45_RS02155 (JKV45_02150) 435367..436605 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T187479 WP_003113526.1 NZ_CP068678:c432031-431753 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T187479 NZ_CP089870:1956272-1956374 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT187479 WP_003113527.1 NZ_CP068678:c431741-431436 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT187479 NZ_CP089870:c1956381-1956236 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References