Detailed information of TA system TA187361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4261683..4262482
• Replicon: chromosome
• Accession: NZ_CP068591
• Organism: Escherichia coli strain EF7-18-58

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: JMY44_RS20190
• Protein ID: WP_000347266.1
• Coordinates: 4262018..4262482 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: JMY44_RS20185
• Protein ID: WP_001307405.1
• Coordinates: 4261683..4262018 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY44_RS20170	4257468..4258238	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
JMY44_RS20175	4258254..4259588	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JMY44_RS20180	4259963..4261534	+	1572	WP_001273741.1	galactarate dehydratase	-
JMY44_RS20185	4261683..4262018	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JMY44_RS20190	4262018..4262482	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JMY44_RS20195	4262537..4263346	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JMY44_RS20200	4263595..4264875	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JMY44_RS20205	4264898..4265371	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JMY44_RS20210	4265382..4266161	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JMY44_RS20215	4266151..4267029	+	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JMY44_RS20220	4267047..4267481	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4250695..4262482	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T187361 WP_000347266.1 NZ_CP068591:4262018-4262482 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T187361 NZ_CP089789:c4607723-4607412 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT187361 WP_001307405.1 NZ_CP068591:4261683-4262018 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT187361 NZ_CP089789:c4607411-4607121 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7