Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3047719..3048221 Replicon chromosome
Accession NZ_CP068591
Organism Escherichia coli strain EF7-18-58

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JMY44_RS14535 Protein ID WP_000767829.1
Coordinates 3047719..3047973 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JMY44_RS14540 Protein ID WP_001259255.1
Coordinates 3047970..3048221 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY44_RS14505 3042734..3042961 - 228 WP_202763971.1 sulfurtransferase TusA family protein -
JMY44_RS14510 3042975..3044033 - 1059 WP_000492330.1 transport protein YeeE -
JMY44_RS14515 3044212..3045570 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JMY44_RS14520 3045560..3045622 - 63 WP_010723108.1 membrane protein YoeI -
JMY44_RS14525 3045837..3046766 - 930 WP_000803351.1 LysR family transcriptional regulator -
JMY44_RS14530 3046812..3047636 - 825 WP_000754737.1 SDR family oxidoreductase -
JMY44_RS14535 3047719..3047973 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JMY44_RS14540 3047970..3048221 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JMY44_RS14545 3048505..3048555 + 51 WP_001364200.1 his operon leader peptide -
JMY44_RS14550 3048701..3049600 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JMY44_RS14555 3049606..3050910 + 1305 WP_001446927.1 histidinol dehydrogenase -
JMY44_RS14560 3050907..3051977 + 1071 WP_000108933.1 histidinol-phosphate transaminase -
JMY44_RS14565 3051977..3053044 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T187357 WP_000767829.1 NZ_CP068591:c3047973-3047719 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T187357 NZ_CP089789:3419630-3419848 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT187357 WP_001259255.1 NZ_CP068591:c3048221-3047970 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT187357 NZ_CP089789:3419228-3419602 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References