Detailed information of TA system TA187342

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2319997..2320368
• Replicon: chromosome
• Accession: NZ_CP068591
• Organism: Escherichia coli strain EF7-18-58

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JMY44_RS10975
• Protein ID: WP_001317028.1
• Coordinates: 2320174..2320368 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2319997..2320175 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY44_RS10945	2315749..2315922	+	174	WP_001296046.1	hypothetical protein	-
JMY44_RS10950	2315952..2317325	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
JMY44_RS10955	2317454..2318389	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JMY44_RS10960	2318441..2319676	-	1236	WP_000040852.1	site-specific integrase	-
JMY44_RS10965	2319678..2319893	-	216	WP_000079604.1	excisionase XisR	-
JMY44_RS10970	2319972..2320181	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2319997..2320175	+	179	NuclAT_0	-	Antitoxin
-	2319997..2320175	+	179	NuclAT_0	-	Antitoxin
-	2319997..2320175	+	179	NuclAT_0	-	Antitoxin
-	2319997..2320175	+	179	NuclAT_0	-	Antitoxin
JMY44_RS10975	2320174..2320368	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JMY44_RS10980	2320425..2321234	-	810	WP_000166319.1	recombination protein RecT	-
JMY44_RS10985	2321227..2323827	-	2601	WP_001532611.1	exodeoxyribonuclease VIII	-
JMY44_RS10990	2323929..2324204	-	276	WP_000632297.1	protein RacC	-
JMY44_RS10995	2324279..2324449	-	171	WP_001352098.1	conserved protein, Rac prophage	-
JMY44_RS11000	2324449..2324670	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2318441..2349008	30567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T187342 WP_001317028.1 NZ_CP068591:c2320368-2320174 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T187342 NZ_CP089788:c734140-733724 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT187342 NZ_CP068591:2319997-2320175 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin