Detailed information of TA system TA187322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 29860..30517
• Replicon: plasmid unnamed1
• Accession: NZ_CP068573
• Organism: Klebsiella pneumoniae strain IR1251

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JJV77_RS26225
• Protein ID: WP_000270043.1
• Coordinates: 29860..30210 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJV77_RS26230
• Protein ID: WP_000124640.1
• Coordinates: 30215..30517 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJV77_RS26180	25171..25350	+	180	Protein_33	helix-turn-helix transcriptional regulator	-
JJV77_RS26185	25415..26119	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JJV77_RS26190	26334..26831	-	498	WP_000062185.1	hypothetical protein	-
JJV77_RS26195	26834..27322	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
JJV77_RS26200	27419..27754	+	336	WP_000683476.1	hypothetical protein	-
JJV77_RS26205	27769..28239	-	471	WP_001281821.1	hypothetical protein	-
JJV77_RS26210	28232..28603	-	372	WP_000516916.1	hypothetical protein	-
JJV77_RS26215	28614..28808	-	195	WP_000343597.1	hypothetical protein	-
JJV77_RS26220	29149..29697	-	549	WP_001061574.1	transcriptional regulator	-
JJV77_RS26225	29860..30210	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJV77_RS26230	30215..30517	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JJV77_RS26235	30544..30837	-	294	WP_001239997.1	chromosome segregation protein ParM	-
JJV77_RS26240	30925..31197	-	273	WP_001043047.1	HU family DNA-binding protein	-
JJV77_RS26245	31255..31782	-	528	WP_001236377.1	thermonuclease family protein	-
JJV77_RS26250	32013..32870	-	858	WP_001167032.1	hypothetical protein	-
JJV77_RS26255	32857..33087	-	231	WP_000972663.1	hypothetical protein	-
JJV77_RS26260	33087..33605	-	519	WP_000210756.1	nitrite reductase	-
JJV77_RS26265	33602..34048	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
JJV77_RS26270	34048..34407	-	360	WP_000422768.1	hypothetical protein	-
JJV77_RS26275	34464..34892	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / sul1 / qacE / dfrA25 / blaCMY-2	-	1..185118	185118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T187322 WP_000270043.1 NZ_CP068573:29860-30210 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T187322 NZ_CP089781:2855362-2855775 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT187322 WP_000124640.1 NZ_CP068573:30215-30517 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT187322 NZ_CP089781:2855814-2856071 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9