Detailed information of TA system TA187259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2637741..2638390
• Replicon: chromosome
• Accession: NZ_CP068551
• Organism: Pseudomonas khazarica strain ODT-83

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLK41_RS12650
• Protein ID: WP_047588122.1
• Coordinates: 2638046..2638390 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLK41_RS12645
• Protein ID: WP_124148941.1
• Coordinates: 2637741..2638049 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLK41_RS12620 (JLK41_12620)	2632844..2633158	-	315	WP_014851536.1	c-type cytochrome	-
JLK41_RS12625 (JLK41_12625)	2633446..2634030	-	585	WP_080757027.1	TSUP family transporter	-
JLK41_RS12630 (JLK41_12630)	2634111..2635316	+	1206	WP_196250622.1	chromate efflux transporter	-
JLK41_RS12635 (JLK41_12635)	2635327..2635632	+	306	WP_047588115.1	PadR family transcriptional regulator	-
JLK41_RS12640 (JLK41_12640)	2635795..2637225	+	1431	WP_210391153.1	IS1182-like element ISPa7 family transposase	-
JLK41_RS12645 (JLK41_12645)	2637741..2638049	-	309	WP_124148941.1	transcriptional regulator	Antitoxin
JLK41_RS12650 (JLK41_12650)	2638046..2638390	-	345	WP_047588122.1	toxin	Toxin
JLK41_RS12655 (JLK41_12655)	2638548..2639474	-	927	WP_210391154.1	LysR family transcriptional regulator	-
JLK41_RS12660 (JLK41_12660)	2639488..2640687	-	1200	WP_210391155.1	MFS transporter	-
JLK41_RS12665 (JLK41_12665)	2640773..2642626	-	1854	WP_210391156.1	asparagine synthase (glutamine-hydrolyzing)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2595232..2672813	77581
	-	flank	IS/Tn	-	-	2635795..2637225	1430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-92)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13366.33 Da
• Isoelectric Point: 10.2892

>T187259 WP_047588122.1 NZ_CP068551:c2638390-2638046 [Pseudomonas khazarica]
MKALFVELSPFERNRKAHLDDDEYSLFQQMLLTNPEAGAVIANSGGLRKVRFGSVRRNKGKRGGVRVIYYYWLRGLQFWL
FTLYDKDELDDLSSDQRRQLKALLEREVKARQAP

Nucleotide

• Length: 345 bp

>T187259 NZ_CP089780:c2364634-2364200 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCGCAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11579.35 Da
• Isoelectric Point: 10.0926

>AT187259 WP_124148941.1 NZ_CP068551:c2638049-2637741 [Pseudomonas khazarica]
MTKRDIFSELIEGFDDLAAAREGKRTLRTQKVEYKPIEPATGAEIVALREKLQMSQGVFAQAIHAKPATLKNWEQNRSKP
NDQATVLIRILNKHPDLIRELA

Nucleotide

• Length: 309 bp

>AT187259 NZ_CP089780:c2364895-2364641 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG