Detailed information of TA system TA187241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1781641..1782301
• Replicon: chromosome
• Accession: NZ_CP068444
• Organism: Klebsiella quasipneumoniae strain SWMUF35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IT777_RS08615
• Protein ID: WP_049004534.1
• Coordinates: 1781948..1782301 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IT777_RS08610
• Protein ID: WP_101995649.1
• Coordinates: 1781641..1781943 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT777_RS08585	1776988..1778091	-	1104	WP_101995648.1	phosphotransferase	-
IT777_RS08595	1778614..1780050	+	1437	WP_023290315.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
IT777_RS08605	1780414..1781346	+	933	Protein_1678	integrase arm-type DNA-binding domain-containing protein	-
IT777_RS08610	1781641..1781943	-	303	WP_101995649.1	XRE family transcriptional regulator	Antitoxin
IT777_RS08615	1781948..1782301	-	354	WP_049004534.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IT777_RS08620	1783121..1787020	+	3900	WP_117129038.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13493.39 Da
• Isoelectric Point: 9.5699

>T187241 WP_049004534.1 NZ_CP068444:c1782301-1781948 [Klebsiella quasipneumoniae]
VWTIKTTDTFDRWFASLNDTDRVSVLAALLVLREKGPGLSRPYADTVRGSRYSNMKELRIQSRGEPIRAFFAFDLTRTGI
VLCAGNKVGNEKRFYDEMLPVADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T187241 NZ_CP089780:c1229820-1229509 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11139.95 Da
• Isoelectric Point: 6.4738

>AT187241 WP_101995649.1 NZ_CP068444:c1781943-1781641 [Klebsiella quasipneumoniae]
MRRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT187241 NZ_CP089780:c1230140-1229820 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A