Detailed information of TA system TA187238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 467746..468467
• Replicon: plasmid unnamed3
• Accession: NZ_CP068443
• Organism: Pantoea agglomerans strain ZJU23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8X8J2A8
• Locus tag: JK642_RS23010
• Protein ID: WP_010247063.1
• Coordinates: 467746..468057 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JK642_RS23015
• Protein ID: WP_031590724.1
• Coordinates: 468054..468467 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK642_RS23000 (JK642_22940)	464449..465741	-	1293	WP_010247061.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
JK642_RS23005 (JK642_22945)	465752..467305	-	1554	WP_235765354.1	aspartate aminotransferase family protein	-
JK642_RS23010 (JK642_22950)	467746..468057	+	312	WP_010247063.1	type II toxin-antitoxin system HigB family toxin	Toxin
JK642_RS23015 (JK642_22955)	468054..468467	+	414	WP_031590724.1	helix-turn-helix domain-containing protein	Antitoxin
JK642_RS23020 (JK642_22960)	468583..469956	-	1374	WP_031590723.1	PTS ascorbate transporter subunit IIC	-
JK642_RS23025 (JK642_22965)	469996..470268	-	273	WP_003855084.1	PTS sugar transporter subunit IIB	-
JK642_RS23030 (JK642_22970)	470317..470766	-	450	WP_069026758.1	PTS sugar transporter subunit IIA	-
JK642_RS23035 (JK642_22975)	471343..472077	+	735	WP_163794180.1	GntR family transcriptional regulator	-
JK642_RS23040 (JK642_22980)	472461..472949	+	489	WP_235765355.1	type VI secretion system tube protein TssD	-
JK642_RS23045 (JK642_22985)	472949..473311	+	363	WP_071941147.1	DUF2778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..567588	567588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12207.12 Da
• Isoelectric Point: 9.8340

>T187238 WP_010247063.1 NZ_CP068443:467746-468057 [Pantoea agglomerans]
MHIISRGPFREAAIIYPNHASALDAAYLVLRDENFATPDALKIRFQSLDRMKYREKWWVIDVGGNSLRIMFYADFDRGKI
FIKHIVTHAEYDKLVKKYRESKE

Nucleotide

• Length: 312 bp

>T187238 NZ_CP089780:c754757-754320 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15420.44 Da
• Isoelectric Point: 4.9795

>AT187238 WP_031590724.1 NZ_CP068443:468054-468467 [Pantoea agglomerans]
MITEAIQATNDLVRIVPFLGGSTDKRDYEQALELVEYLVEHQPDSPLVEILSDKVARYENSAPEFAAFNARTDAMPRGVA
LLRVIMDQHGLTQSSFADEIGQRSYVSRILRGDRPLTDKHKARLAARFNLPFEAFAE

Nucleotide

• Length: 414 bp

>AT187238 NZ_CP089780:c755008-754754 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA