Detailed information of TA system TA187224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1588864..1589489
• Replicon: chromosome
• Accession: NZ_CP068435
• Organism: Cupriavidus necator isolate XXL

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: JJQ59_RS26325
• Protein ID: WP_114130693.1
• Coordinates: 1588864..1589142 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJQ59_RS26330
• Protein ID: WP_035815276.1
• Coordinates: 1589154..1589489 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJQ59_RS26325	1588864..1589142	+	279	WP_114130693.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJQ59_RS26330	1589154..1589489	+	336	WP_035815276.1	HigA family addiction module antidote protein	Antitoxin
JJQ59_RS26335	1589540..1590910	-	1371	WP_114130692.1	serine hydrolase	-
JJQ59_RS26340	1590957..1591436	-	480	WP_013953223.1	MarR family transcriptional regulator	-
JJQ59_RS26345	1591828..1592322	+	495	WP_202281558.1	hypothetical protein	-
JJQ59_RS26350	1592371..1592685	+	315	WP_114136428.1	transposase	-
JJQ59_RS26355	1592616..1593284	+	669	WP_202281706.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10685.32 Da
• Isoelectric Point: 10.7650

>T187224 WP_114130693.1 NZ_CP068435:1588864-1589142 [Cupriavidus necator]
MIQSFRCSDTAELFAGRCVPRFGNIRTVAERKLRLLQRASTLEFLRSPPGNRLQLLTGDRKGRYSIRINEQWRICFIWTH
DGPAAVEIIDNH

Nucleotide

• Length: 279 bp

>T187224 NZ_CP089780:361937-362239 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12275.07 Da
• Isoelectric Point: 8.6214

>AT187224 WP_035815276.1 NZ_CP068435:1589154-1589489 [Cupriavidus necator]
VVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYD
LKVAQRDHGERIMAEVAPRNAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT187224 NZ_CP089780:362287-362508 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA