Detailed information of TA system TA187209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4400784..4401386
• Replicon: chromosome
• Accession: NZ_CP068394
• Organism: Escherichia coli strain M-17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: JK368_RS21145
• Protein ID: WP_000897302.1
• Coordinates: 4400784..4401095 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JK368_RS21150
• Protein ID: WP_000356397.1
• Coordinates: 4401096..4401386 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK368_RS21120	4396698..4397297	+	600	WP_001314324.1	glucose-1-phosphatase	-
JK368_RS21125	4397291..4398163	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JK368_RS21130	4398160..4398597	+	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JK368_RS21135	4398642..4399583	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JK368_RS21140	4399647..4400555	-	909	WP_001331553.1	alpha/beta hydrolase	-
JK368_RS21145	4400784..4401095	+	312	WP_000897302.1	hypothetical protein	Toxin
JK368_RS21150	4401096..4401386	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JK368_RS21155	4401745..4402023	+	279	WP_001296612.1	hypothetical protein	-
JK368_RS21160	4402419..4402637	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
JK368_RS21165	4402853..4403782	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JK368_RS21170	4403779..4404414	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JK368_RS21175	4404411..4405313	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T187209 WP_000897302.1 NZ_CP068394:4400784-4401095 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T187209 NZ_CP089779:c3537762-3537355 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT187209 WP_000356397.1 NZ_CP068394:4401096-4401386 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT187209 NZ_CP089779:c3538019-3537759 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: S1P416