Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3587103..3587902 Replicon chromosome
Accession NZ_CP068394
Organism Escherichia coli strain M-17

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JK368_RS17215 Protein ID WP_000347251.1
Coordinates 3587438..3587902 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag JK368_RS17210 Protein ID WP_001296435.1
Coordinates 3587103..3587438 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK368_RS17195 3582888..3583658 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JK368_RS17200 3583674..3585008 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
JK368_RS17205 3585383..3586954 + 1572 WP_001273939.1 galactarate dehydratase -
JK368_RS17210 3587103..3587438 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JK368_RS17215 3587438..3587902 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JK368_RS17220 3587957..3588766 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JK368_RS17225 3589015..3590295 + 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
JK368_RS17230 3590318..3590791 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
JK368_RS17235 3590802..3591581 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JK368_RS17240 3591571..3592449 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JK368_RS17245 3592467..3592901 + 435 WP_000948834.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T187208 WP_000347251.1 NZ_CP068394:3587438-3587902 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T187208 NZ_CP089779:3502144-3502527 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT187208 WP_001296435.1 NZ_CP068394:3587103-3587438 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187208 NZ_CP089779:3501894-3502049 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References