Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1535805..1536358 Replicon chromosome
Accession NZ_CP068394
Organism Escherichia coli strain M-17

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag JK368_RS07485 Protein ID WP_000605680.1
Coordinates 1536089..1536358 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JK368_RS07480 Protein ID WP_000781370.1
Coordinates 1535805..1536089 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK368_RS07465 1530986..1534033 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
JK368_RS07470 1534046..1534930 + 885 WP_046960541.1 formate dehydrogenase N subunit beta -
JK368_RS07475 1534923..1535576 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
JK368_RS07480 1535805..1536089 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JK368_RS07485 1536089..1536358 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JK368_RS07490 1536544..1537554 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
JK368_RS07495 1537688..1539385 - 1698 WP_000433464.1 malate dehydrogenase -
JK368_RS07500 1539542..1539679 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JK368_RS07505 1539781..1539996 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JK368_RS07510 1540341..1540772 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T187193 WP_000605680.1 NZ_CP068394:c1536358-1536089 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T187193 NZ_CP089779:2309141-2309530 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT187193 WP_000781370.1 NZ_CP068394:c1536089-1535805 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT187193 NZ_CP089779:2308917-2309144 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References