Detailed information of TA system TA187135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30539..31269
• Replicon: chromosome
• Accession: NZ_CP068287
• Organism: Enterobacter hormaechei strain 1575

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: JJL14_RS00155
• Protein ID: WP_022646552.1
• Coordinates: 30539..30853 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJL14_RS00160
• Protein ID: WP_023305145.1
• Coordinates: 30850..31269 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL14_RS00130	26621..27706	+	1086	Protein_25	cellulase family glycosylhydrolase	-
JJL14_RS00135	27612..28796	-	1185	WP_023298963.1	multidrug efflux MFS transporter EmrD	-
JJL14_RS00140	28972..29389	-	418	Protein_27	EamA family transporter	-
JJL14_RS00145	29452..29898	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
JJL14_RS00150	29963..30052	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
JJL14_RS00155	30539..30853	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
JJL14_RS00160	30850..31269	+	420	WP_023305145.1	helix-turn-helix domain-containing protein	Antitoxin
JJL14_RS00165	31416..31514	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
JJL14_RS00170	31621..33309	+	1689	WP_063148199.1	acetolactate synthase large subunit	-
JJL14_RS00175	33313..33600	+	288	WP_008500144.1	acetolactate synthase small subunit	-
JJL14_RS00180	33726..34319	+	594	WP_022646555.1	transcriptional regulator UhpA	-
JJL14_RS00185	34316..35821	+	1506	WP_048966212.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T187135 WP_022646552.1 NZ_CP068287:30539-30853 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T187135 NZ_CP089778:c2202447-2202118 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.15 Da
• Isoelectric Point: 5.6761

>AT187135 WP_023305145.1 NZ_CP068287:30850-31269 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT187135 NZ_CP089778:c2202821-2202444 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A155SSD8