Detailed information of TA system TA187127

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54803..55224
• Replicon: plasmid pCY598
• Accession: NZ_CP068284
• Organism: Escherichia coli strain CY598

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JI738_RS23180
• Protein ID: WP_096937776.1
• Coordinates: 54803..54928 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	55026..55224 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI738_RS23145 (50200)	50200..50889	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
JI738_RS23150 (51076)	51076..51459	-	384	WP_001151527.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JI738_RS23155 (51792)	51792..52382	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
JI738_RS23160 (52678)	52678..53499	-	822	WP_001234417.1	DUF932 domain-containing protein	-
JI738_RS23165 (53618)	53618..53905	-	288	WP_000107542.1	hypothetical protein	-
JI738_RS23170 (53930)	53930..54136	-	207	WP_000547965.1	hypothetical protein	-
JI738_RS23175 (54206)	54206..54502	+	297	Protein_62	hypothetical protein	-
JI738_RS23180 (54803)	54803..54928	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
JI738_RS23410 (54870)	54870..55019	-	150	Protein_64	DUF5431 family protein	-
- (55026)	55026..55224	-	199	NuclAT_0	-	Antitoxin
- (55026)	55026..55224	-	199	NuclAT_0	-	Antitoxin
- (55026)	55026..55224	-	199	NuclAT_0	-	Antitoxin
- (55026)	55026..55224	-	199	NuclAT_0	-	Antitoxin
JI738_RS23185 (55193)	55193..55955	-	763	Protein_65	plasmid SOS inhibition protein A	-
JI738_RS23190 (55952)	55952..56386	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
JI738_RS23195 (56441)	56441..58399	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
JI738_RS23200 (58465)	58465..58698	-	234	WP_000005990.1	DUF905 family protein	-
JI738_RS23205 (58755)	58755..59294	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67408	67408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T187127 WP_096937776.1 NZ_CP068284:c54928-54803 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T187127 NZ_CP089778:1384573-1385004 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT187127 NZ_CP068284:c55224-55026 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin