Detailed information of TA system TA187124

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16965..17219
• Replicon: plasmid pCY598
• Accession: NZ_CP068284
• Organism: Escherichia coli strain CY598

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JI738_RS22950
• Protein ID: WP_001312851.1
• Coordinates: 16965..17114 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	17158..17219 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI738_RS22915 (12407)	12407..12553	-	147	Protein_10	3'-5' exonuclease	-
JI738_RS22920 (12808)	12808..12977	-	170	Protein_11	resolvase	-
JI738_RS22925 (12973)	12973..13857	-	885	WP_001345827.1	hypothetical protein	-
JI738_RS22930 (14791)	14791..15648	-	858	WP_000774301.1	incFII family plasmid replication initiator RepA	-
JI738_RS22935 (15641)	15641..16123	-	483	WP_001443034.1	hypothetical protein	-
JI738_RS22940 (16116)	16116..16190	-	75	WP_001442103.1	RepA leader peptide Tap	-
JI738_RS22945 (16424)	16424..16681	-	258	WP_000083834.1	replication regulatory protein RepA	-
JI738_RS22950 (16965)	16965..17114	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (17158)	17158..17219	+	62	NuclAT_1	-	Antitoxin
- (17158)	17158..17219	+	62	NuclAT_1	-	Antitoxin
- (17158)	17158..17219	+	62	NuclAT_1	-	Antitoxin
- (17158)	17158..17219	+	62	NuclAT_1	-	Antitoxin
JI738_RS22955 (17515)	17515..17826	+	312	WP_000802277.1	hypothetical protein	-
JI738_RS22960 (18007)	18007..18093	-	87	Protein_19	endonuclease	-
JI738_RS22965 (18291)	18291..18479	-	189	WP_001345829.1	hypothetical protein	-
JI738_RS22970 (18614)	18614..19174	-	561	WP_000139310.1	fertility inhibition protein FinO	-
JI738_RS22975 (19229)	19229..19975	-	747	WP_000205776.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67408	67408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T187124 WP_001312851.1 NZ_CP068284:c17114-16965 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T187124 NZ_CP089778:1167756-1168637 [Mycobacterium tuberculosis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Nucleotide

• Length: 62 bp

>AT187124 NZ_CP068284:17158-17219 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin