Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3335089..3335888 Replicon chromosome
Accession NZ_CP068283
Organism Escherichia coli strain CY598

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JI738_RS16405 Protein ID WP_000347273.1
Coordinates 3335424..3335888 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JI738_RS16400 Protein ID WP_001307405.1
Coordinates 3335089..3335424 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI738_RS16385 (3330874) 3330874..3331644 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
JI738_RS16390 (3331660) 3331660..3332994 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JI738_RS16395 (3333369) 3333369..3334940 + 1572 WP_001273753.1 galactarate dehydratase -
JI738_RS16400 (3335089) 3335089..3335424 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JI738_RS16405 (3335424) 3335424..3335888 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JI738_RS16410 (3335943) 3335943..3336752 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JI738_RS16415 (3337001) 3337001..3338281 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JI738_RS16420 (3338304) 3338304..3338777 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JI738_RS16425 (3338788) 3338788..3339159 + 372 Protein_3218 PTS sugar transporter subunit IIC -
JI738_RS16430 (3339155) 3339155..3339712 + 558 Protein_3219 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3325941..3346872 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T187113 WP_000347273.1 NZ_CP068283:3335424-3335888 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T187113 NZ_CP089778:c698696-698283 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT187113 WP_001307405.1 NZ_CP068283:3335089-3335424 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187113 NZ_CP089778:c698929-698693 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References