Detailed information of TA system TA187078

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66247..66668
• Replicon: plasmid pCY706
• Accession: NZ_CP068282
• Organism: Escherichia coli strain CY706

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJW04_RS23110
• Protein ID: WP_096937776.1
• Coordinates: 66543..66668 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66247..66445 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW04_RS23085 (62177)	62177..62716	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
JJW04_RS23090 (62773)	62773..63006	+	234	WP_000005990.1	DUF905 family protein	-
JJW04_RS23095 (63072)	63072..65030	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
JJW04_RS23100 (65085)	65085..65519	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
JJW04_RS23105 (65516)	65516..66278	+	763	Protein_76	plasmid SOS inhibition protein A	-
- (66247)	66247..66445	+	199	NuclAT_0	-	Antitoxin
- (66247)	66247..66445	+	199	NuclAT_0	-	Antitoxin
- (66247)	66247..66445	+	199	NuclAT_0	-	Antitoxin
- (66247)	66247..66445	+	199	NuclAT_0	-	Antitoxin
JJW04_RS23280 (66452)	66452..66601	+	150	Protein_77	DUF5431 family protein	-
JJW04_RS23110 (66543)	66543..66668	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJW04_RS23115 (66969)	66969..67265	-	297	Protein_79	hypothetical protein	-
JJW04_RS23120 (67335)	67335..67541	+	207	WP_000547965.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67545	67545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T187078 WP_096937776.1 NZ_CP068282:66543-66668 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T187078 NZ_CP089777:c2218874-2218578 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT187078 NZ_CP068282:66247-66445 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin