Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1460490..1461184 Replicon chromosome
Accession NZ_CP068281
Organism Escherichia coli strain CY706

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JJW04_RS06935 Protein ID WP_001263489.1
Coordinates 1460786..1461184 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JJW04_RS06930 Protein ID WP_000554758.1
Coordinates 1460490..1460783 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW04_RS06910 (1456122) 1456122..1456619 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJW04_RS06915 (1456843) 1456843..1458555 - 1713 Protein_1340 flagellar biosynthesis protein FlhA -
JJW04_RS06920 (1458527) 1458527..1459312 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JJW04_RS06925 (1459383) 1459383..1460438 + 1056 WP_001226164.1 DNA polymerase IV -
JJW04_RS06930 (1460490) 1460490..1460783 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JJW04_RS06935 (1460786) 1460786..1461184 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JJW04_RS06940 (1461194) 1461194..1461646 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
JJW04_RS06945 (1461964) 1461964..1462170 + 207 Protein_1346 RtcB family protein -
JJW04_RS06950 (1462166) 1462166..1462687 + 522 Protein_1347 peptide chain release factor H -
JJW04_RS06955 (1462744) 1462744..1464201 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JJW04_RS06960 (1464462) 1464462..1464920 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1465516) 1465516..1465596 + 81 NuclAT_12 - -
- (1465516) 1465516..1465596 + 81 NuclAT_12 - -
- (1465516) 1465516..1465596 + 81 NuclAT_12 - -
- (1465516) 1465516..1465596 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T187043 WP_001263489.1 NZ_CP068281:1460786-1461184 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T187043 NZ_CP089776:c3697469-3697041 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT187043 WP_000554758.1 NZ_CP068281:1460490-1460783 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT187043 NZ_CP089776:c3697715-3697473 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References