Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4426245..4427044 Replicon chromosome
Accession NZ_CP068279
Organism Escherichia coli strain CY708

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JJW05_RS21500 Protein ID WP_000347273.1
Coordinates 4426580..4427044 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJW05_RS21495 Protein ID WP_001307405.1
Coordinates 4426245..4426580 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW05_RS21480 (4422030) 4422030..4422800 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
JJW05_RS21485 (4422816) 4422816..4424150 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJW05_RS21490 (4424525) 4424525..4426096 + 1572 WP_001273753.1 galactarate dehydratase -
JJW05_RS21495 (4426245) 4426245..4426580 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJW05_RS21500 (4426580) 4426580..4427044 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJW05_RS21505 (4427099) 4427099..4427908 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJW05_RS21510 (4428157) 4428157..4429437 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJW05_RS21515 (4429460) 4429460..4429933 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJW05_RS21520 (4429944) 4429944..4430315 + 372 Protein_4205 PTS sugar transporter subunit IIC -
JJW05_RS21525 (4430311) 4430311..4430868 + 558 Protein_4206 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4417097..4438028 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T187028 WP_000347273.1 NZ_CP068279:4426580-4427044 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T187028 NZ_CP089776:2800354-2800779 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT187028 WP_001307405.1 NZ_CP068279:4426245-4426580 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187028 NZ_CP089776:2800127-2800348 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References