Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4382971..4383698 Replicon chromosome
Accession NZ_CP068279
Organism Escherichia coli strain CY708

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JJW05_RS21275 Protein ID WP_000550189.1
Coordinates 4383384..4383698 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JJW05_RS21270 Protein ID WP_000560266.1
Coordinates 4382971..4383387 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW05_RS21260 (4378131) 4378131..4380482 + 2352 WP_000695487.1 alpha-glucosidase -
JJW05_RS21265 (4380908) 4380908..4382926 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JJW05_RS21270 (4382971) 4382971..4383387 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JJW05_RS21275 (4383384) 4383384..4383698 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JJW05_RS21280 (4383982) 4383982..4385118 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JJW05_RS21285 (4385203) 4385203..4385706 + 504 WP_001333820.1 M48 family metallopeptidase -
JJW05_RS21290 (4385783) 4385783..4386475 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JJW05_RS21295 (4386554) 4386554..4387540 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T187027 WP_000550189.1 NZ_CP068279:c4383698-4383384 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T187027 NZ_CP089776:c2397238-2396921 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT187027 WP_000560266.1 NZ_CP068279:c4383387-4382971 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT187027 NZ_CP089776:c2397450-2397235 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References