Detailed information of TA system TA187002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1406348..1407042
• Replicon: chromosome
• Accession: NZ_CP068279
• Organism: Escherichia coli strain CY708

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: JJW05_RS06670
• Protein ID: WP_001263489.1
• Coordinates: 1406644..1407042 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JJW05_RS06665
• Protein ID: WP_000554758.1
• Coordinates: 1406348..1406641 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW05_RS06645 (1401980)	1401980..1402477	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
JJW05_RS06650 (1402701)	1402701..1404413	-	1713	Protein_1291	flagellar biosynthesis protein FlhA	-
JJW05_RS06655 (1404385)	1404385..1405170	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
JJW05_RS06660 (1405241)	1405241..1406296	+	1056	WP_001226164.1	DNA polymerase IV	-
JJW05_RS06665 (1406348)	1406348..1406641	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJW05_RS06670 (1406644)	1406644..1407042	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJW05_RS06675 (1407052)	1407052..1407504	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
JJW05_RS06680 (1407822)	1407822..1408028	+	207	Protein_1297	RtcB family protein	-
JJW05_RS06685 (1408024)	1408024..1408545	+	522	Protein_1298	peptide chain release factor H	-
JJW05_RS06690 (1408602)	1408602..1410059	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
JJW05_RS06695 (1410320)	1410320..1410778	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (1411374)	1411374..1411454	+	81	NuclAT_11	-	-
- (1411374)	1411374..1411454	+	81	NuclAT_11	-	-
- (1411374)	1411374..1411454	+	81	NuclAT_11	-	-
- (1411374)	1411374..1411454	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T187002 WP_001263489.1 NZ_CP068279:1406644-1407042 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T187002 NZ_CP089776:c761468-761160 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT187002 WP_000554758.1 NZ_CP068279:1406348-1406641 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT187002 NZ_CP089776:c761700-761455 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3