Detailed information of TA system TA186903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 60100..60746
• Replicon: chromosome
• Accession: NZ_CP068234
• Organism: Leclercia adecarboxylata strain YZ30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JJN11_RS00310
• Protein ID: WP_103177685.1
• Coordinates: 60396..60746 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJN11_RS00305
• Protein ID: WP_156653669.1
• Coordinates: 60100..60399 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN11_RS00280 (JJN11_00280)	55833..56921	-	1089	WP_156653667.1	AAA family ATPase	-
JJN11_RS00285 (JJN11_00285)	57066..58025	-	960	WP_103177681.1	DUF523 and DUF1722 domain-containing protein	-
JJN11_RS00290 (JJN11_00290)	58128..58538	-	411	WP_114385495.1	hydroxyisourate hydrolase	-
JJN11_RS00295 (JJN11_00295)	58635..59363	-	729	WP_156653668.1	MerR family transcriptional regulator	-
JJN11_RS00300 (JJN11_00300)	59481..60005	+	525	WP_103177683.1	lipocalin family protein	-
JJN11_RS00305 (JJN11_00305)	60100..60399	-	300	WP_156653669.1	XRE family transcriptional regulator	Antitoxin
JJN11_RS00310 (JJN11_00310)	60396..60746	-	351	WP_103177685.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJN11_RS00315 (JJN11_00315)	60857..61294	+	438	WP_156653670.1	acetyltransferase	-
JJN11_RS00320 (JJN11_00320)	61367..61978	-	612	WP_231736573.1	hypothetical protein	-
JJN11_RS00325 (JJN11_00325)	62217..62504	-	288	WP_156653671.1	SymE family type I addiction module toxin	-
JJN11_RS00330 (JJN11_00330)	62633..62809	+	177	WP_156653672.1	YlcI/YnfO family protein	-
JJN11_RS00335 (JJN11_00335)	62854..63546	-	693	WP_156653673.1	nucleotidyltransferase domain-containing protein	-
JJN11_RS00340 (JJN11_00340)	63543..64745	-	1203	WP_156653674.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13547.46 Da
• Isoelectric Point: 7.2761

>T186903 WP_103177685.1 NZ_CP068234:c60746-60396 [Leclercia adecarboxylata]
MWTVILGEVFEAWLEDQEQGVQEKVFAALINLQTYGPRLPRPYADTVRGSRHNNMKELRIQYYGRPIRAFFAFDPVRQAI
VLCAGDKSHDKKFYERLIRIADDEFSAHLAAMETRN

Nucleotide

• Length: 351 bp

>T186903 NZ_CP089774:c2223238-2222819 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCATGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11195.12 Da
• Isoelectric Point: 8.6049

>AT186903 WP_156653669.1 NZ_CP068234:c60399-60100 [Leclercia adecarboxylata]
MKTLNEAIASRSPESQKRIKEMADEMILETGLQMMREELKLSQKHVAEVMGISQPAITQLEQRGNELKITTLKRYIEAMG
GKLSLDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT186903 NZ_CP089774:c2223507-2223247 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA