Detailed information of TA system TA186869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3123043..3123718
• Replicon: chromosome
• Accession: NZ_CP068215
• Organism: Enterobacter hormaechei strain FDAARGOS_1078

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7T9SGM4
• Locus tag: I6I35_RS15010
• Protein ID: WP_032654718.1
• Coordinates: 3123043..3123342 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I35_RS15015
• Protein ID: WP_032654721.1
• Coordinates: 3123353..3123718 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I35_RS15000	3119420..3121612	+	2193	WP_032654716.1	type I secretion system permease/ATPase	-
I6I35_RS15005	3121632..3122792	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
I6I35_RS15010	3123043..3123342	+	300	WP_032654718.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I35_RS15015	3123353..3123718	+	366	WP_032654721.1	HigA family addiction module antidote protein	Antitoxin
I6I35_RS15020	3123745..3124926	-	1182	WP_017382758.1	PLP-dependent aminotransferase family protein	-
I6I35_RS15025	3124947..3125834	-	888	WP_032654725.1	LysR family transcriptional regulator	-
I6I35_RS15030	3125932..3126534	+	603	WP_032654728.1	short chain dehydrogenase	-
I6I35_RS15035	3126531..3127262	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	3119420..3173338	53918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-71)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11582.25 Da
• Isoelectric Point: 9.8425

>T186869 WP_032654718.1 NZ_CP068215:3123043-3123342 [Enterobacter hormaechei]
MINHYRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SAGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T186869 NZ_CP089773:3823280-3823690 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13731.81 Da
• Isoelectric Point: 9.5936

>AT186869 WP_032654721.1 NZ_CP068215:3123353-3123718 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKLGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT186869 NZ_CP089773:3823005-3823283 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A7T9SGM4