Detailed information of TA system TA186858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 672958..673685
• Replicon: chromosome
• Accession: NZ_CP068215
• Organism: Enterobacter hormaechei strain FDAARGOS_1078

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3V067
• Locus tag: I6I35_RS03310
• Protein ID: WP_022650547.1
• Coordinates: 673374..673685 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I35_RS03305
• Protein ID: WP_032652900.1
• Coordinates: 672958..673377 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I35_RS03290	670029..671150	-	1122	WP_032652893.1	glutamate--cysteine ligase	-
I6I35_RS03295	671256..672146	+	891	WP_032652895.1	oxidoreductase	-
I6I35_RS03300	672200..672634	-	435	WP_032652897.1	hypothetical protein	-
I6I35_RS03305	672958..673377	-	420	WP_032652900.1	helix-turn-helix domain-containing protein	Antitoxin
I6I35_RS03310	673374..673685	-	312	WP_022650547.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6I35_RS03315	674378..674797	+	420	WP_032655562.1	hypothetical protein	-
I6I35_RS03320	674911..675366	-	456	WP_180244858.1	hypothetical protein	-
I6I35_RS03325	675657..676538	-	882	WP_003858850.1	LysR family transcriptional regulator	-
I6I35_RS03330	676644..677423	+	780	WP_026094386.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12554.34 Da
• Isoelectric Point: 9.9435

>T186858 WP_022650547.1 NZ_CP068215:c673685-673374 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T186858 NZ_CP089773:c3072173-3071757 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15437.48 Da
• Isoelectric Point: 4.6051

>AT186858 WP_032652900.1 NZ_CP068215:c673377-672958 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT186858 NZ_CP089773:c3072436-3072170 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A8B3V067