Detailed information of TA system TA186853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4334569..4335299
• Replicon: chromosome
• Accession: NZ_CP068214
• Organism: Serratia ureilytica strain FDAARGOS_1089

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: I6I46_RS20845
• Protein ID: WP_004937033.1
• Coordinates: 4334569..4334880 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I46_RS20850
• Protein ID: WP_033644489.1
• Coordinates: 4334877..4335299 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I46_RS20815	4329884..4330609	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
I6I46_RS20820	4330662..4332095	+	1434	WP_129993438.1	RNA polymerase factor sigma-54	-
I6I46_RS20825	4332119..4332406	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
I6I46_RS20830	4332562..4333023	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
I6I46_RS20835	4333151..4334005	+	855	WP_015379177.1	RNase adapter RapZ	-
I6I46_RS20840	4334002..4334274	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
I6I46_RS20845	4334569..4334880	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6I46_RS20850	4334877..4335299	+	423	WP_033644489.1	helix-turn-helix domain-containing protein	Antitoxin
I6I46_RS20855	4335306..4337222	-	1917	WP_033644486.1	BglG family transcription antiterminator	-
I6I46_RS20860	4337274..4338488	-	1215	WP_033644485.1	lactonase family protein	-
I6I46_RS20865	4338576..4339316	-	741	WP_033644484.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T186853 WP_004937033.1 NZ_CP068214:4334569-4334880 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T186853 NZ_CP089773:2870944-2871357 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCAAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT186853 WP_033644489.1 NZ_CP068214:4334877-4335299 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT186853 NZ_CP089773:2871396-2871653 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63