Detailed information of TA system TA186851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4253403..4254181
• Replicon: chromosome
• Accession: NZ_CP068214
• Organism: Serratia ureilytica strain FDAARGOS_1089

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6I46_RS20395
• Protein ID: WP_060453218.1
• Coordinates: 4253864..4254181 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I46_RS20390
• Protein ID: WP_060453219.1
• Coordinates: 4253403..4253738 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I46_RS20375	4249588..4250403	-	816	WP_125460772.1	GPO family capsid scaffolding protein	-
I6I46_RS20380	4250546..4252318	+	1773	WP_201910984.1	terminase ATPase subunit family protein	-
I6I46_RS20385	4252318..4253352	+	1035	WP_033649034.1	phage portal protein	-
I6I46_RS20390	4253403..4253738	-	336	WP_060453219.1	HigA family addiction module antidote protein	Antitoxin
I6I46_RS20395	4253864..4254181	-	318	WP_060453218.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I46_RS20400	4254609..4254806	-	198	WP_126186641.1	hypothetical protein	-
I6I46_RS20405	4254854..4255078	-	225	WP_164119617.1	hypothetical protein	-
I6I46_RS20410	4255117..4257333	-	2217	WP_201910985.1	replication endonuclease	-
I6I46_RS20415	4257330..4257722	-	393	WP_201910986.1	hypothetical protein	-
I6I46_RS20420	4257715..4257993	-	279	WP_197779752.1	DUF5405 family protein	-
I6I46_RS20425	4258003..4258176	-	174	WP_201911162.1	hypothetical protein	-
I6I46_RS20430	4258116..4258340	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
I6I46_RS20435	4258340..4258651	-	312	WP_126502297.1	DUF5405 family protein	-
I6I46_RS20440	4258651..4258893	-	243	WP_023447523.1	DUF2732 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	-	4226504..4266686	40182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.91 Da
• Isoelectric Point: 9.9499

>T186851 WP_060453218.1 NZ_CP068214:c4254181-4253864 [Serratia ureilytica]
VQLTDKKKSIESFRDQWLDDFFVTGKGHKKIPPDIGIPLGRKLDIINSAVSHKDLRSPPGNRFEKLNPPLGEYCSIRVNG
QYRLIFKWIDGKAVDLYLDNHTYRA

Nucleotide

• Length: 318 bp

>T186851 NZ_CP089773:2811100-2811525 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12926.78 Da
• Isoelectric Point: 4.8782

>AT186851 WP_060453219.1 NZ_CP068214:c4253738-4253403 [Serratia ureilytica]
MVQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTVSALVNNNRKLTVDMAFRLAKAFDTTEQFWLNLQLNLDLW
EIQNDARIQEELNRVITMENFLEKREHPVAA

Nucleotide

• Length: 336 bp

>AT186851 NZ_CP089773:2810873-2811094 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA