Detailed information of TA system TA186829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 52904..53555
• Replicon: plasmid unnamed4
• Accession: NZ_CP068205
• Organism: Acinetobacter johnsonii strain FDAARGOS_1092

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: I6I49_RS01375
• Protein ID: WP_004668307.1
• Coordinates: 52904..53260 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I49_RS01380
• Protein ID: WP_001140620.1
• Coordinates: 53253..53555 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I49_RS01365	49516..50244	-	729	Protein_46	HNH endonuclease	-
I6I49_RS01370	51013..52347	+	1335	WP_201949948.1	ISNCY family transposase	-
I6I49_RS01375	52904..53260	+	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I49_RS01380	53253..53555	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
I6I49_RS01390	54730..55902	+	1173	WP_201949955.1	MFS transporter	-
I6I49_RS01395	55997..56893	+	897	WP_201949956.1	LysR family transcriptional regulator	-
I6I49_RS01400	57071..58042	+	972	WP_005399577.1	LLM class oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131816	131816
	-	inside	IScluster/Tn	-	-	51013..54406	3393

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T186829 WP_004668307.1 NZ_CP068205:52904-53260 [Acinetobacter johnsonii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T186829 NZ_CP089773:1019790-1020224 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT186829 WP_001140620.1 NZ_CP068205:53253-53555 [Acinetobacter johnsonii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT186829 NZ_CP089773:1019605-1019784 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: N9NM16