Detailed information of TA system TA186812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 94521..95106
• Replicon: plasmid unnamed1
• Accession: NZ_CP068175
• Organism: Acinetobacter ursingii strain FDAARGOS_1096

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BUL4
• Locus tag: I6I53_RS00465
• Protein ID: WP_004942805.1
• Coordinates: 94786..95106 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I53_RS00460
• Protein ID: WP_004942828.1
• Coordinates: 94521..94793 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I53_RS00430	89947..91226	-	1280	Protein_85	ISNCY family transposase	-
I6I53_RS00435	91374..91802	-	429	WP_004999914.1	arsenate reductase (glutaredoxin)	-
I6I53_RS00440	91887..92210	+	324	WP_004999912.1	metalloregulator ArsR/SmtB family transcription factor	-
I6I53_RS00445	92216..92689	+	474	WP_004942838.1	arsenate reductase ArsC	-
I6I53_RS00450	92694..93734	+	1041	WP_004999910.1	ACR3 family arsenite efflux transporter	-
I6I53_RS00455	93739..94443	+	705	WP_004942830.1	arsenical resistance protein ArsH	-
I6I53_RS00460	94521..94793	-	273	WP_004942828.1	helix-turn-helix domain-containing protein	Antitoxin
I6I53_RS00465	94786..95106	-	321	WP_004942805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I53_RS00470	95288..96555	+	1268	Protein_93	ISNCY family transposase	-
I6I53_RS00475	96804..97781	-	978	WP_004999903.1	oxidoreductase	-
I6I53_RS00480	97865..98509	-	645	WP_004942792.1	TetR/AcrR family transcriptional regulator	-
I6I53_RS00485	98689..99635	+	947	Protein_96	IS5 family transposase	-
I6I53_RS00490	99681..99986	-	306	Protein_97	acyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120510	120510
	-	inside	IScluster/Tn	-	-	87324..103880	16556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12270.05 Da
• Isoelectric Point: 7.9834

>T186812 WP_004942805.1 NZ_CP068175:c95106-94786 [Acinetobacter ursingii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLFQPDKGDLIKNGGGIRKVRCTQGNKGKSGGIRVIYYWVNEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T186812 NZ_CP089773:367952-368254 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10119.58 Da
• Isoelectric Point: 8.6582

>AT186812 WP_004942828.1 NZ_CP068175:c94793-94521 [Acinetobacter ursingii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT186812 NZ_CP089773:367728-367955 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7T9UFL0