Detailed information of TA system TA186810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2287031..2287601
• Replicon: chromosome
• Accession: NZ_CP068173
• Organism: Brevibacterium casei strain FDAARGOS_1100

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K9APH7
• Locus tag: I6I57_RS09990
• Protein ID: WP_009374925.1
• Coordinates: 2287031..2287312 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I57_RS09995
• Protein ID: WP_009374927.1
• Coordinates: 2287299..2287601 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I57_RS09975	2283103..2285658	+	2556	WP_063249396.1	DEAD/DEAH box helicase family protein	-
I6I57_RS09980	2285724..2286533	+	810	WP_009374923.1	hypothetical protein	-
I6I57_RS09985	2286704..2286913	-	210	WP_040340933.1	hypothetical protein	-
I6I57_RS09990	2287031..2287312	+	282	WP_009374925.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I57_RS09995	2287299..2287601	+	303	WP_009374927.1	HigA family addiction module antidote protein	Antitoxin
I6I57_RS10000	2287605..2288153	+	549	WP_144587898.1	GNAT family N-acetyltransferase	-
I6I57_RS10005	2288217..2288723	+	507	WP_009374931.1	hypothetical protein	-
I6I57_RS10010	2288960..2289256	+	297	WP_063251004.1	hypothetical protein	-
I6I57_RS10015	2289316..2289585	-	270	WP_040340935.1	DUF3263 domain-containing protein	-
I6I57_RS10020	2289744..2290889	-	1146	WP_144587894.1	glycerol dehydrogenase	-
I6I57_RS10025	2291213..2292445	+	1233	WP_156491205.1	low temperature requirement protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2277344..2288723	11379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10785.21 Da
• Isoelectric Point: 7.2802

>T186810 WP_009374925.1 NZ_CP068173:2287031-2287312 [Brevibacterium casei]
VIRSFGSKETERLWRREHVPSLDPRIQRVALRKLRQVGSAEVLEDLSVPPGNRLEALKGDRAGQHSIRINDQWRICFVWT
DAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T186810 NZ_CP089772:3818461-3818871 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11002.56 Da
• Isoelectric Point: 5.0747

>AT186810 WP_009374927.1 NZ_CP068173:2287299-2287601 [Brevibacterium casei]
MTTTDKIEPIHPGEVLMKDFIEGFGITQNKLAVAIGVPPRRINEIVHGNRGISADTALRLAKYFGTSAEFWINLQSHYEL
DRAEDLAGEQIAAITPLQVA

Nucleotide

• Length: 303 bp

>AT186810 NZ_CP089772:3818186-3818464 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: K9APH7