Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2909826..2910553 Replicon chromosome
Accession NZ_CP068155
Organism Escherichia coli strain FDAARGOS_1077

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag I6I34_RS15210 Protein ID WP_000550189.1
Coordinates 2909826..2910140 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6I34_RS15215 Protein ID WP_000560266.1
Coordinates 2910137..2910553 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I34_RS15190 2905984..2906970 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
I6I34_RS15195 2907049..2907741 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
I6I34_RS15200 2907818..2908321 - 504 WP_001333820.1 M48 family metallopeptidase -
I6I34_RS15205 2908406..2909542 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
I6I34_RS15210 2909826..2910140 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
I6I34_RS15215 2910137..2910553 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
I6I34_RS15220 2910598..2912616 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
I6I34_RS15225 2912842..2915193 - 2352 WP_000695470.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T186783 WP_000550189.1 NZ_CP068155:2909826-2910140 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T186783 NZ_CP089772:c2196031-2195702 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT186783 WP_000560266.1 NZ_CP068155:2910137-2910553 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT186783 NZ_CP089772:c2196405-2196028 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References