Detailed information of TA system TA186771

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34829..35093
• Replicon: plasmid unnamed1
• Accession: NZ_CP068153
• Organism: Escherichia coli strain FDAARGOS_1077

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: I6I34_RS00230
• Protein ID: WP_001303307.1
• Coordinates: 34941..35093 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	34829..34891 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I34_RS00215	30068..32359	-	2292	WP_077738502.1	conjugal transfer protein TrbC	-
I6I34_RS00220	32352..33422	-	1071	WP_052895276.1	IncI1-type conjugal transfer protein TrbB	-
I6I34_RS00225	33441..34649	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	34829..34891	-	63	NuclAT_0	-	Antitoxin
-	34829..34891	-	63	NuclAT_0	-	Antitoxin
-	34829..34891	-	63	NuclAT_0	-	Antitoxin
-	34829..34891	-	63	NuclAT_0	-	Antitoxin
I6I34_RS00230	34941..35093	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
I6I34_RS00235	35165..35416	-	252	WP_001291965.1	hypothetical protein	-
-	35803..35854	-	52	NuclAT_1	-	-
-	35803..35854	-	52	NuclAT_1	-	-
-	35803..35854	-	52	NuclAT_1	-	-
-	35803..35854	-	52	NuclAT_1	-	-
I6I34_RS00240	36340..36516	-	177	WP_001054900.1	hypothetical protein	-
I6I34_RS00245	36908..37117	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
I6I34_RS00250	37189..37851	-	663	WP_077738432.1	plasmid IncI1-type surface exclusion protein ExcA	-
I6I34_RS00255	37916..40084	-	2169	WP_077738433.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80453	80453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T186771 WP_001303307.1 NZ_CP068153:34941-35093 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T186771 NZ_CP089772:1016904-1017338 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT186771 NZ_CP068153:c34891-34829 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin