Detailed information of TA system TA186757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 101903..102654
• Replicon: plasmid unnamed
• Accession: NZ_CP068149
• Organism: Serratia liquefaciens strain FDAARGOS_1081

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A380D7R5
• Locus tag: I6I38_RS25315
• Protein ID: WP_017891116.1
• Coordinates: 101903..102214 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I38_RS25320
• Protein ID: WP_170868577.1
• Coordinates: 102253..102654 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I38_RS25295	97326..97832	+	507	WP_201896505.1	antirestriction protein	-
I6I38_RS25300	98514..100556	+	2043	WP_201896506.1	ParB/RepB/Spo0J family partition protein	-
I6I38_RS25305	100598..101062	+	465	WP_201896507.1	conjugation system SOS inhibitor PsiB	-
I6I38_RS25310	101059..101787	+	729	WP_128865572.1	plasmid SOS inhibition protein A	-
I6I38_RS25315	101903..102214	+	312	WP_017891116.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6I38_RS25320	102253..102654	+	402	WP_170868577.1	helix-turn-helix domain-containing protein	Antitoxin
I6I38_RS25325	103218..103670	+	453	WP_128865574.1	hypothetical protein	-
I6I38_RS25330	103731..103880	+	150	WP_165366321.1	hypothetical protein	-
I6I38_RS25335	104327..105145	+	819	WP_201896508.1	DUF945 domain-containing protein	-
I6I38_RS25340	105193..105495	+	303	WP_128865576.1	hypothetical protein	-
I6I38_RS25345	105627..105989	-	363	WP_142106025.1	mobilization protein MobC	-
I6I38_RS25350	106237..106542	+	306	WP_017891124.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pilW / pilW	1..187192	187192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12245.20 Da
• Isoelectric Point: 10.1438

>T186757 WP_017891116.1 NZ_CP068149:101903-102214 [Serratia liquefaciens]
VHVISRQPFNEAAGKYPNSALALLDLMRVLEKKTFNSPDEMKRAIPSLDNFKYRKKWWVIDVSGNTLRLMAFIDFEKQKV
FVKHIATHAEYDKLTKYYREHKE

Nucleotide

• Length: 312 bp

>T186757 NZ_CP089772:c448740-448147 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAAAAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14708.84 Da
• Isoelectric Point: 4.6655

>AT186757 WP_170868577.1 NZ_CP068149:102253-102654 [Serratia liquefaciens]
MIATFANAVKCIPLMTGDKSEAEYKKALELIEYLVDRDELSNPLFEPLAAKITEYENSAPEFEAFNRRLAEIPTGVAALR
TLMDQHGLKAADLEDELGSKSNVSNILSGRRALTVQHIKALATRFDVPADLFI

Nucleotide

• Length: 402 bp

>AT186757 NZ_CP089772:c449158-448769 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A380D7R5